Journal: Frontiers in Cellular Neuroscience

Article Title: Reprogramming of adult human dermal fibroblasts to induced dorsal forebrain precursor cells maintains aging signatures

doi: 10.3389/fncel.2023.1003188

Figure Lengend Snippet: Phase contrast images of (A) HDFs and (B) resulting hiDFP colonies from 20/30-, 50- and 70-year-old subjects. Scale = 300 μm. HDF, human dermal fibroblast; hiDFP, human induced dorsal forebrain precursor.

Article Snippet: Nine adult human dermal fibroblast cell lines (HDFs) were obtained from Cell Applications Inc or The NINDS Human Cell and Data Repository.

Techniques:

Journal: Frontiers in Cellular Neuroscience

Article Title: Reprogramming of adult human dermal fibroblasts to induced dorsal forebrain precursor cells maintains aging signatures

doi: 10.3389/fncel.2023.1003188

Figure Lengend Snippet: Reprogrammed hiDFPs maintain aging-associated features. (A) SA-βgal activity assay of HDFs and hiDFPs. No statistical significance was determined by two-way mixed ANOVA. (B) Mitochondrial superoxide levels in HDFs and hiDFPs. Data represent mean ± SEM with n = 3. Simple main effect of reprogramming on mitochondrial superoxide levels determined by two-way mixed ANOVA with * for p < 0.05.

Article Snippet: Nine adult human dermal fibroblast cell lines (HDFs) were obtained from Cell Applications Inc or The NINDS Human Cell and Data Repository.

Techniques: Activity Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: Reprogramming of adult human dermal fibroblasts to induced dorsal forebrain precursor cells maintains aging signatures

doi: 10.3389/fncel.2023.1003188

Figure Lengend Snippet: Reprogrammed hiDFPs exhibit increased amounts of DNA methylation as measured by the production of 5-methylcytosine (5-mC). (A) Amount of methylated DNA in hiPSCs and hiDFPs relative to HDF. Significance was determined by one-way ANOVA with Tukey post-hoc test. (B) Amount of methylated DNA in HDFs and hiDFPs. A simple main effect of reprogramming irrespective of age group was determined by two-way mixed ANOVA. Data represent mean ± SEM with n = 3. * for p < 0.05, ** for p ≤ 0.01.

Article Snippet: Nine adult human dermal fibroblast cell lines (HDFs) were obtained from Cell Applications Inc or The NINDS Human Cell and Data Repository.

Techniques: DNA Methylation Assay, Methylation

Journal: Gels

Article Title: Streamlining Skin Regeneration: A Ready-To-Use Silk Bilayer Wound Dressing

doi: 10.3390/gels10070439

Figure Lengend Snippet: ( A ) Rehydration of the sterilized dry scaffold and reversion of SS layer to a hydrogel-like structure before cell seeding; ( B ) seeding efficiency on the bilayer and SS structures; ( C ) cell metabolic activity of HDFs seeded on the bilayer and SS structures; ( D ) DNA content on the bilayer constructs. Asterisks correspond to statistically significant differences (*** p < 0.001, ** p < 0.01).

Article Snippet: Human dermal fibroblasts (HDFs) were sourced from Lonza (catalog #CC-2509) and cultivated as a monolayer under standard conditions of 37 °C and 5% CO2.

Techniques: Activity Assay, Construct

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: The Induction of Apoptosis in A375 Malignant Melanoma Cells by Sutherlandia frutescens

doi: 10.1155/2016/4921067

Figure Lengend Snippet: The effect of S. frutescens extract on the viability of (a) A375 melanoma, (b) Colo-800 melanoma, (c) HDF α fibroblast, and (d) Hek 293 cells after 24, 48, and 72 h of treatment. The viability was assessed using the alamarBlue assay and expressed as percentage relative to the control-treated cells (0 mg/mL). Error bars represent the SEM ( n ≥ 3) and ∗ indicates a significant difference from 0 mg/mL ( P < 0.05).

Article Snippet: The human primary dermal fibroblast cell line (HDF α ) was purchased from ScienCell, CA, USA.

Techniques: Alamar Blue Assay, Control